Biology Vitamin C - 2056 Words

Investigating the vitamin C content of fruits and fruit juice Aim: In this investigation, I aim to measure and compare whether there is a higher content of vitamin C in a fruit or a juice by measuring the volume of the sample required to decolourise a solution of dichlorophenolindophenol (DCPIP). Introduction: For this experiment, I aim to investigate and compare the content of vitamin C in the fruit against the fruit juice. The theory of this method is a titration with dichlorophenolindophenol (DCPIP). DCPIP is a redox dye and Ascorbic acid reacts with the DCPIP solution, changing the colour from a deep blue to either a pale pink or colourless solution. They react in a 1:1 state, so I can use the amount of ascorbic acid required to work†¦show more content†¦The amount of DPCPIP solution used throughout the whole experiment will be kept constant, 5ml at a time. This is to prevent any errors or complications when working out the averages. Variables: My dependant variable is the indicator, DCPIP, and the independent variables are both the grapefruit juice, and grapefruit. Risk assessment amp; factors that can affect my experiment: Knife safety – is slightly sharp so use with care Wear goggles in case any of the grapefruit squirts in your eye whilst cutting Take care with fragile glassware Ascorbic acid is flammable, if inhaled could cause a sore throat. Irritant to skin and eyes for some. The solutions may stain your skin and clothes so protective clothing will be useful. Main apparatus: Quantity: | Apparatus: | Why and how it is used: | 45ml | DCPIP solution | It is a good indicator for vitamin C because if it’s present, the blue dye is reduced to a colourless compound by ascorbic acid.DCPIP reacts with ascorbic acid to turn colourless. When this occurs, the fruit juice being added will be stopped and noted down. | 10mg | Ascorbic acid | Also known as vitamin C. Is the dependant variable, so can be used as a focal point to see how much vitamin C is in a grapefruit and grapefruit juice. It will be used to see how much pure vitamin C is needed to turn the DCPIP indicator colourless. When this happens, the amount of fruit juice used will be recorded. | 150mg | Grapefruit juice |Show MoreRelatedBiology Vitamin C Lab Report4458 Words   |  18 PagesBIOLOGY LAB REPORT TITLE: DETERMINING THE VITAMIN C CONTENT IN VARIOUS FRUIT JUICE PREPARED BY: NOR HUSNA BINTI MOHD ROSLI CLASS: SC3 (ALUK 11) IC NUMBER: STUDENT ID: LECTURER’S NAME: MADAM ZAKIAH BINTI ZAKARIA SUBMISSION DATE: 22 AUGUST 2013 OBJECTIVES: To investigate the vitamin C content in various fruit juices which is based on the graph’s curve obtained. 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Hussein, Dep.of chemistry, college of science, Babylon University Muhammed K.Alasedi Ministry of Health Hilla city, Babylon Abstract The goal of this search is to determine the concentration of vitamin C (Ascorbic acid ) in some of a Commercial Products, Vegetables and Fruit Juices by Redox Titration. A redoxRead MoreThe Effect Of Vitamin C On Concentration Essay3257 Words   |  14 PagesAS91387 VITAMIN-C CONCENTRATION Purpose The purpose of this investigation was to study the effects of vitamin C decreasing in concentration over time after being exposed to oxygen. This occurs by the ascorbic acid (which is the organic molecule known as vitamin C) breaking down in the process of oxygen in an oxidation reaction. Enzymes in the citrus fruits increase the rate of oxidation. A deaeration step minimizes amount of oxygen so the amount of ascorbic acid lost is minimized. â€Å"Vitamin C, alsoRead More Linus Pauling Essay3903 Words   |  16 Pagessignificant contributions including his work on the nature of the chemical bond, he turned to chemical biology and is generally accepted as the founder of molecular biology. Later in his life he became very involved in issues of politics and peace for which he is somewhat less well known. In his later years, he became interested in health and medicine and specifically in the use of vitamin C to prevent ailments from the common cold to cancer. In Pauling’s own words he was â€Å"†¦a physicist